Chromatography experiments

Chromatography is one of the chemical experimental techniques used to separate and analyze the components of a mixture. It is based on the principle that a substance is distributed between a fixed phase (static phase) and a mobile phase (mobile phase). There are many different types of chromatography, with gas chromatography (GC) and liquid chromatography (LC) being two of the most commonly used.

Gas Chromatography (GC) Experiments: Equipment and materials:

Gas Chromatography Equipment: Column:

Chromatography column of choice based on the compound you want to analyze Carrier Gas:

Gases used in analysis (nitrogen is typically used) Sample:

Samples containing the mixture to be analyzed Standard Material:

Standards to validate results or measure quantity Experimental Procedure:

Sample Note

separation

As the sample passes through the column, it separates from the inside of the column. It is based on the principle that each component is adsorbed and released differently.

Detect

Components that pass through the column are detected by the detector, from which data is collected.

Data analysis

The collected data is used to analyze and interpret the amount and properties of each ingredient.

Liquid Chromatography (LC) Experiments: Equipment & Materials

Liquid Chromatography Sheet

Column

The chromatog of your choice based on the compound you want to analyze Mobile Phase (Elluent):

Liquids used in analysis

Stasis (Stationary Phase):

Coated material on the inside of the column Detector:

A device for detecting isolated components Experimental Procedure:

Sample Injection

Separation:

As the sample passes through the column, it interacts with the stagnant phase inside the column and separates.

Detect:

The isolated components are detected by the detector, from which data is collected.

Data Analysis:

Use the collected data to analyze and interpret the quantities and properties of each ingredient Chromatographic experiments are used extensively for the separation and analysis of compounds, and are of particular importance in the food and drug industries, for example. Prior to performing an experiment, it is important to familiarize yourself with safety regulations and instructions for using the equipment, and to make precise adjustments to the equipment to obtain accurate data.